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TroponinT-GCaMP5-Zeo
(Plasmid #46027)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46027 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    TroponinT-Gateway
  • Total vector size (bp) 10565
  • Modifications to backbone
    GCaMP5-2A-Zeo cloned into TroponinT-Gateway using LR Recombinase.
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP5-2A-Zeo
  • Species
    Synthetic
  • Insert Size (bp)
    1800
  • Promoter TNNT2

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Please note that Addgene's sequencing results identified nucleotide mismatches at bp# 3408, 4035, 4136 & 4641 when compared to the full plasmid sequence. According to the depositing laboratory, these differences are not a concern for the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TroponinT-GCaMP5-Zeo was a gift from John Gearhart (Addgene plasmid # 46027 ; http://n2t.net/addgene:46027 ; RRID:Addgene_46027)
  • For your References section:

    Optimization of direct fibroblast reprogramming to cardiomyocytes using calcium activity as a functional measure of success. Addis RC, Ifkovits JL, Pinto F, Kellam LD, Esteso P, Rentschler S, Christoforou N, Epstein JA, Gearhart JD. J Mol Cell Cardiol. 2013 Apr 13;60C:97-106. doi: 10.1016/j.yjmcc.2013.04.004. 10.1016/j.yjmcc.2013.04.004 PubMed 23591016