|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46137||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8938
- Total vector size (bp) 10260
Vector typeInsect Expression
Growth in Bacteria
Alt nameGAL4 inhibitor GAL80
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)1322
Entrez GeneGAL80 (a.k.a. YML051W)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer Pry1
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Please see this additional reference https://www.ncbi.nlm.nih.gov/pubmed/20434990 for more information on the Q-system.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQUAST-GAL80 was a gift from Christopher Potter (Addgene plasmid # 46137 ; http://n2t.net/addgene:46137 ; RRID:Addgene_46137)
For your References section:Improved and expanded Q-system reagents for genetic manipulations. Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ. Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250. 10.1038/nmeth.3250 PubMed 25581800