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pFT1.0
(Plasmid #46138)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46138 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript II KS+
  • Backbone manufacturer
    stratagene
  • Total vector size (bp) 6131
  • Vector type
    Cre/Lox ; mutagenic gene trap for zebrafish

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    3900

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site DraIII (unknown if destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer mCherryR
  • 3′ sequencing primer M13 F20
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

mCherry is in the "even" or "0" reading frame starting from the exon.
This is a Tol2-based FlExTrap vector. It consists of a highly mutagenic gene trap contained within the FlEx cassette that can be stably inverted by both Cre and Flp recombinases. FRT/F3 and loxP/lox5171 are incompatible recombination sites used for the Flp and Cre recombinases, respectively, to stably invert the cassette. The reporter gene, mCherry, has its own initiation codon.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFT1.0 was a gift from Wenbiao Chen (Addgene plasmid # 46138 ; http://n2t.net/addgene:46138 ; RRID:Addgene_46138)
  • For your References section:

    Conditional control of gene function by an invertible gene trap in zebrafish. Ni TT, Lu J, Zhu M, Maddison LA, Boyd KL, Huskey L, Ju B, Hesselson D, Zhong TP, Page-McCaw PS, Stainier DY, Chen W. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15389-94. Epub 2012 Aug 20. 10.1073/pnas.1206131109 PubMed 22908272