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pET15b/GgVcl V1-258 (aka VD1)
(Plasmid #46173)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46173 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    EMD
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 6485
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vcl 1-258
  • Alt name
    Vinculin
  • Alt name
    Vcl
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    780
  • Mutation
    aa 1-258 (VD1)
  • GenBank ID
    NM_205441.1
  • Entrez Gene
    VCL
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b/GgVcl V1-258 (aka VD1) was a gift from Susan Craig (Addgene plasmid # 46173 ; http://n2t.net/addgene:46173 ; RRID:Addgene_46173)
  • For your References section:

    Two distinct head-tail interfaces cooperate to suppress activation of vinculin by talin. Cohen DM, Chen H, Johnson RP, Choudhury B, Craig SW. J Biol Chem. 2005 Apr 29;280(17):17109-17. Epub 2005 Feb 22. 10.1074/jbc.M414704200 PubMed 15728584