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pET15b/GgVcl 1-851 A50I mutation
(Plasmid #46174)


Item Catalog # Description Quantity Price (USD)
Plasmid 46174 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 8264
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Vcl 1-851 A50I
  • Alt name
  • Alt name
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
  • Mutation
    aa 1-851 A50I mutation (talin binding domain mutant in vinculin head domain)
  • GenBank ID
  • Entrez Gene
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

See Addgene plasmid 46172 for wild-type cloning information. The A50I mutation was introduced by QuikChange mutagenesis (Stratagene).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b/GgVcl 1-851 A50I mutation was a gift from Susan Craig (Addgene plasmid # 46174 ; ; RRID:Addgene_46174)
  • For your References section:

    A conformational switch in vinculin drives formation and dynamics of a talin-vinculin complex at focal adhesions. Cohen DM, Kutscher B, Chen H, Murphy DB, Craig SW. J Biol Chem. 2006 Jun 9;281(23):16006-15. Epub 2006 Apr 10. 10.1074/jbc.M600738200 PubMed 16608855