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pEGFPC1/GgVcl 1-1066 T12 mutant
(Plasmid #46266)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46266 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clonetech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7990
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Vcl T12 mutant
  • Alt name
    Vinculin
  • Alt name
    Vcl
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    3259
  • Mutation
    T12 mutant (D974;K975;R976; R978A) autoinhibition decreased by 100x
  • GenBank ID
    NM_205441.1
  • Entrez Gene
    VCL
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

T12 mutation introduced by QuikChange PCR to Addgene plasmid 46265.

Mutant inhibits vinculin autoinhibition by ~ 100 fold, vinculin is partially activated and will bind talin, but not actin (unless both talin and actin are present at same time).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPC1/GgVcl 1-1066 T12 mutant was a gift from Susan Craig (Addgene plasmid # 46266 ; http://n2t.net/addgene:46266 ; RRID:Addgene_46266)
  • For your References section:

    Two distinct head-tail interfaces cooperate to suppress activation of vinculin by talin. Cohen DM, Chen H, Johnson RP, Choudhury B, Craig SW. J Biol Chem. 2005 Apr 29;280(17):17109-17. Epub 2005 Feb 22. 10.1074/jbc.M414704200 PubMed 15728584