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pGW_GPHN.FingR-YFP
(Plasmid #46298)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46298 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGW_YFP
  • Backbone size w/o insert (bp) 5541
  • Total vector size (bp) 5860
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GPHN.FingR
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    318
  • Entrez Gene
    GPHN (a.k.a. GEPH, GPH, GPHRYN, HKPX1, MOCODC)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CTTAGGCACAGCACG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGW_GPHN.FingR-YFP was a gift from Don Arnold (Addgene plasmid # 46298 ; http://n2t.net/addgene:46298 ; RRID:Addgene_46298)
  • For your References section:

    Recombinant probes for visualizing endogenous synaptic proteins in living neurons. Gross GG, Junge JA, Mora RJ, Kwon HB, Olson CA, Takahashi TT, Liman ER, Ellis-Davies GC, McGee AW, Sabatini BL, Roberts RW, Arnold DB. Neuron. 2013 Jun 19;78(6):971-85. doi: 10.1016/j.neuron.2013.04.017. 10.1016/j.neuron.2013.04.017 PubMed 23791193