|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46303||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 5925
- Total vector size (bp) 8350
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameLrrc3b mouse monoclonal antibody (low affinity)
Alt nameleucine rich repeat containing 3B
SpeciesD. rerio (zebrafish)
Insert Size (bp)2400
Entrez Genelrrc3b (a.k.a. zgc:162270)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer M13 rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypTT3 was obtained from Yves Durocher, National Research Council of Canada - Biotechnology Research Institute
Terms and Licenses
Please note that Addgene's sequencing results contain a few nucleotide mismatches when compared to the sequence provided by the depositing laboratory. These mismatches do not affect the production of recombinant antibodies, nor the affinity of the antibody for its antigen.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SI13-Lrrc3b was a gift from Gavin Wright (Addgene plasmid # 46303 ; http://n2t.net/addgene:46303 ; RRID:Addgene_46303)
For your References section:A rapid and scalable method for selecting recombinant mouse monoclonal antibodies. Crosnier C, Staudt N, Wright GJ. BMC Biol. 2010 . 8():76. 10.1186/1741-7007-8-76 PubMed 20525357