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pGL3-B-pCR
(Plasmid #46369)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46369 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 5500
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    calretinin promoter
  • Alt name
    pCR
  • Alt name
    CALB2
  • Alt name
    calbindin 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    770
  • Mutation
    promoter
  • Entrez Gene
    CALB2 (a.k.a. CAB29, CAL2, CR)
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer REVprimer3
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-B-pCR was a gift from Rolf Stahel (Addgene plasmid # 46369 ; http://n2t.net/addgene:46369 ; RRID:Addgene_46369)
  • For your References section:

    GAS5 long non-coding RNA in malignant pleural mesothelioma. Renganathan A, Kresoja-Rakic J, Echeverry N, Ziltener G, Vrugt B, Opitz I, Stahel RA, Felley-Bosco E. Mol Cancer. 2014 May 23;13:119. doi: 10.1186/1476-4598-13-119. 10.1186/1476-4598-13-119 PubMed 24885398