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pHT-trxA.ads
(Plasmid #47383)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47383 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHT01
  • Backbone manufacturer
    Mo Bi Tec
  • Backbone size w/o insert (bp) 7956
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ads
  • Alt name
    amorphadiene synthase
  • Species
    Artemisia annua
  • Insert Size (bp)
    1656
  • GenBank ID
    JQ319661.1
  • Promoter Pgrac
  • Tags / Fusion Proteins
    • trxA (N terminal on insert)
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)
  • 5′ sequencing primer LacI-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHT-trxA.ads was a gift from Heng-Phon Too (Addgene plasmid # 47383 ; http://n2t.net/addgene:47383 ; RRID:Addgene_47383)
  • For your References section:

    Optimization of amorphadiene synthesis in Bacillus subtilis via transcriptional, translational, and media modulation. Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP. Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900. 10.1002/bit.24900 PubMed 23483530