Purposecontains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48162||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6273
- Total vector size (bp) 6362
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name5" UTR of Phb
SpeciesM. musculus (mouse)
GenBank IDNM_008831 NM_008831
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site Nhe1 (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:psicheck-2-Phb was a gift from Vamsi Mootha (Addgene plasmid # 48162 ; http://n2t.net/addgene:48162 ; RRID:Addgene_48162)
For your References section:Upstream open reading frames cause widespread reduction of protein expression and are polymorphic among humans. Calvo SE, Pagliarini DJ, Mootha VK. Proc Natl Acad Sci U S A. 2009 May 5;106(18):7507-12. doi: 10.1073/pnas.0810916106. Epub 2009 Apr 16. 10.1073/pnas.0810916106 PubMed 19372376
Map uploaded by the depositor.