pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48283||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 4710
Vector typeBacterial Expression
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberLow Copy
Terms and Licenses
This plasmid is an empty vector to be used with a LIC cloning protocol.
To clone into this vector, add LIC version 2 fusion tags to the 5' end of your PCR primers.
Forward - 5'TTTAAGAAGGAGATATAGATC3'
Reverse - 5'TTATGGAGTTGGGATCTTATTA3'In addition, ensure that your open reading frame contains an ATG start codon.
Linearize the plasmid with EcoRV and gel purify.
When digesting the DNA with T4 polymerase for LIC version 2, use dGTP for insert and dCTP for vector. Series 9 vectors have BioBrick restriction sites to facilitate subcloning reactions to make polycistronic expression vectors. NotI, PacI, AsiSI, and SbfI are the restriction enzyme sites that flank your open reading frame. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET LIC cloning vector with BioBrick polycistronic restriction sites (9A) was a gift from Scott Gradia (Addgene plasmid # 48283 ; http://n2t.net/addgene:48283 ; RRID:Addgene_48283)