Skip to main content
Holiday Schedule: Addgene will be closed November 25th & 26th for the Thanksgiving Holiday. Order processing and shipping may be delayed during this week. For questions about your shipment please contact [email protected].
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pDONR P2R-P3-V5-6xHis
(Plasmid #48347)


Item Catalog # Description Quantity Price (USD)
Plasmid 48347 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pDONR P2R-P3
  • Backbone manufacturer
    Life Technologies/Invitrogen
  • Backbone size w/o insert (bp) 2635
  • Total vector size (bp) 2735
  • Vector type
    Gateway entry clone

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    V5 and 6xHis epitope tags cassette
  • Species
  • Insert Size (bp)
  • Mutation
    Contains a stop codon at the end of the epitope tags cassette.
  • Promoter none

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 forward (-20)
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Life Technologies
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR P2R-P3-V5-6xHis was a gift from Anna Planas & Tomas Santalucia (Addgene plasmid # 48347 ; ; RRID:Addgene_48347)
  • For your References section:

    A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector. Buj R, Iglesias N, Planas AM, Santalucia T. BMC Mol Biol. 2013 Aug 20;14(1):18. doi: 10.1186/1471-2199-14-18. 10.1186/1471-2199-14-18 PubMed 23957834