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pFRT-TODestFLAGHAhFXR1
(Plasmid #48694)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48694 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Life Technologies, Modified by Tuschl Lab
  • Backbone size w/o insert (bp) 5322
  • Total vector size (bp) 7287
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FlagHA FXR1 (isoform b)
  • Alt name
    FXR1
  • Alt name
    FXR1P
  • Alt name
    fragile X mental retardation, autosomal homolog 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1965
  • GenBank ID
    NM_001013438.2
  • Entrez Gene
    FXR1 (a.k.a. FXR1P)
  • Promoter CMV, 2x Tet
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT-TODestFLAGHAhFXR1 was a gift from Thomas Tuschl (Addgene plasmid # 48694 ; http://n2t.net/addgene:48694 ; RRID:Addgene_48694)
  • For your References section:

    FMRP targets distinct mRNA sequence elements to regulate protein expression. Ascano M Jr, Mukherjee N, Bandaru P, Miller JB, Nusbaum JD, Corcoran DL, Langlois C, Munschauer M, Dewell S, Hafner M, Williams Z, Ohler U, Tuschl T. Nature. 2012 Dec 20;492(7429):382-6. doi: 10.1038/nature11737. Epub 2012 Dec 12. 10.1038/nature11737 PubMed 23235829