p8RwB
(Plasmid #48733)

• Purpose: dsRed plasmid
• Depositing Lab: John Schiller
• Publication: Roberts et al Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.

Backbone

• Vector backbone: custom
• Total vector size (bp): 7828
• Vector type: Mammalian Expression
• Selectable markers: Zeocin, Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dsRed-Express
• Species: Discosoma sp
• Promoter: EF1a

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is nearly identical to pRwB (http://www.addgene.org/48734), except that it contains a 2kb stuffer region to bring its size closer to the ~8kb native papillomavirus genome. For more information on using this plasmid, please see the following website: http://home.ccr.cancer.gov/Lco/target.htm