pBEG R3-miRNA(ccdB)-L4 #BGV228
Purpose(Empty Backbone) To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48953||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBEG R3-L4 (addgene plasmid 48954)
Backbone manufacturerDankort Lab
- Backbone size (bp) 4058
Modifications to backboneA miRNA-30 cassette was synthesized by BioBasic and cloned into the NotI/EcoRV sites of pBEG R3-L4, then an EcoRI-XhoI flanked chloramphenicol ccdB cassette was cloned into the EcoRI-XhoI sites of the miRNA-30 cassette.
Vector typeGateway cloning vector
- Promoter na
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Kanamycin
Growth Strain(s)ccdB Survival
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
Please note that Addgene's sequencing results identified a few sequencing differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing lab, these differences are not a concern for the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBEG R3-miRNA(ccdB)-L4 #BGV228 was a gift from David Dankort (Addgene plasmid # 48953 ; http://n2t.net/addgene:48953 ; RRID:Addgene_48953)
For your References section:A modular lentiviral and retroviral construction system to rapidly generate vectors for gene expression and gene knockdown in vitro and in vivo. Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, Dankort D. PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279. 10.1371/journal.pone.0076279 PubMed 24146852
Map uploaded by Addgene staff.