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pBEG R3-miRNA(ccdB)-L4 #BGV228
(Plasmid #48953)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48953 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBEG R3-L4 (addgene plasmid 48954)
  • Backbone manufacturer
    Dankort Lab
  • Backbone size (bp) 4058
  • Modifications to backbone
    A miRNA-30 cassette was synthesized by BioBasic and cloned into the NotI/EcoRV sites of pBEG R3-L4, then an EcoRI-XhoI flanked chloramphenicol ccdB cassette was cloned into the EcoRI-XhoI sites of the miRNA-30 cassette.
  • Vector type
    Gateway cloning vector
  • Promoter na

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Please note that Addgene's sequencing results identified a few sequencing differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositing lab, these differences are not a concern for the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBEG R3-miRNA(ccdB)-L4 #BGV228 was a gift from David Dankort (Addgene plasmid # 48953 ; http://n2t.net/addgene:48953 ; RRID:Addgene_48953)
  • For your References section:

    A modular lentiviral and retroviral construction system to rapidly generate vectors for gene expression and gene knockdown in vitro and in vivo. Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, Dankort D. PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279. 10.1371/journal.pone.0076279 PubMed 24146852