pCheck2 Dest (R1-R2) #BGV129
Purpose(Empty Backbone) Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48955||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerRajewsky Lab, PMID 18327259
Modifications to backboneCreated by blunt end cloning of an attR1-attR2 destination cassette (Invitrogen) into the NotI site (blunted using Klenow) of pSiP1.
Vector typeLuciferase ; Gateway cloning vector
- Promoter SV40
Growth in Bacteria
Bacterial Resistance(s)Chloramphenicol and Ampicillin
Growth Strain(s)ccdB Survival
- Cloning method Gateway Cloning
- 5′ sequencing primer na (Common Sequencing Primers)
The presence of firefly luciferase in the psiCHECK-2-derived vectors allows normalization of the Renilla luciferase expression that monitors the RNAi effect.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCheck2 Dest (R1-R2) #BGV129 was a gift from David Dankort (Addgene plasmid # 48955 ; http://n2t.net/addgene:48955 ; RRID:Addgene_48955)
For your References section:A modular lentiviral and retroviral construction system to rapidly generate vectors for gene expression and gene knockdown in vitro and in vivo. Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, Dankort D. PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279. 10.1371/journal.pone.0076279 PubMed 24146852
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