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pCheck2 Dest (R1-R2) #BGV129
(Plasmid #48955)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 48955 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSiP1
  • Backbone manufacturer
    Rajewsky Lab, PMID 18327259
  • Modifications to backbone
    Created by blunt end cloning of an attR1-attR2 destination cassette (Invitrogen) into the NotI site (blunted using Klenow) of pSiP1.
  • Vector type
    Luciferase ; Gateway cloning vector
  • Promoter SV40

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

Resource Information

Depositor Comments

The presence of firefly luciferase in the psiCHECK-2-derived vectors allows normalization of the Renilla luciferase expression that monitors the RNAi effect.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCheck2 Dest (R1-R2) #BGV129 was a gift from David Dankort (Addgene plasmid # 48955 ; http://n2t.net/addgene:48955 ; RRID:Addgene_48955)
  • For your References section:

    A modular lentiviral and retroviral construction system to rapidly generate vectors for gene expression and gene knockdown in vitro and in vivo. Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, Dankort D. PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279. 10.1371/journal.pone.0076279 PubMed 24146852