pBEG R2-iHygro-L3 #BGV206
PurposeSupplies the selection marker (hygromycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||48985||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBEG R2+xL3 (Addgene plasmid 48952)
Backbone manufacturerDankort Lab
- Total vector size (bp) 3916
Vector typeGateway cloning vector
Growth in Bacteria
Gene/Insert namehygromycin phosphotransferase
Alt namehygromycin resistance
- Promoter na
/ Fusion Protein
- IRES (weak) (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13 R (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBEG R2-iHygro-L3 #BGV206 was a gift from David Dankort (Addgene plasmid # 48985 ; http://n2t.net/addgene:48985 ; RRID:Addgene_48985)
For your References section:A modular lentiviral and retroviral construction system to rapidly generate vectors for gene expression and gene knockdown in vitro and in vivo. Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, Dankort D. PLoS One. 2013 Oct 11;8(10):e76279. doi: 10.1371/journal.pone.0076279. 10.1371/journal.pone.0076279 PubMed 24146852
Map uploaded by Addgene staff.