PurposeExpresses KLF3 in mammalian cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49100||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5446
- Total vector size (bp) 6481
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameKrüppel-Like Factor 3(KLF3)
SpeciesM. musculus (mouse)
Insert Size (bp)1035
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer GAAATTAATACGACTCAC
- 3′ sequencing primer GCGAGCTCTAGCATTTAG (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-KLF3 was a gift from Merlin Crossley (Addgene plasmid # 49100 ; http://n2t.net/addgene:49100 ; RRID:Addgene_49100)
For your References section:Loss of Kruppel-like factor 3 (KLF3/BKLF) leads to upregulation of the insulin-sensitizing factor adipolin (FAM132A/CTRP12/C1qdc2). Bell-Anderson KS, Funnell AP, Williams H, Mat Jusoh H, Scully T, Lim WF, Burdach JG, Mak KS, Knights AJ, Hoy AJ, Nicholas HR, Sainsbury A, Turner N, Pearson RC, Crossley M. Diabetes. 2013 Aug;62(8):2728-37. doi: 10.2337/db12-1745. Epub 2013 Apr 30. 10.2337/db12-1745 PubMed 23633521
Map uploaded by the depositor.