PurposeAAV plasmid with hs1218 promoter driving expression of iCre.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49130||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV
Growth in Bacteria
Copy numberLow Copy
- Promoter hs1218
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer pCAGGS-3
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Additional reference for hs1218 promoter: Visel et al., 2007. VISTA Enhancer Browser--a database of tissue-specific human enhancers. Nucleic Acids Res. 2007 Jan;35(Database issue):D88-92. Epub 2006 Nov 27. PMID: 17130149 (https://www.ncbi.nlm.nih.gov/pubmed/17130149)
Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEMS2023 was a gift from Elizabeth Simpson (Addgene plasmid # 49130 ; http://n2t.net/addgene:49130 ; RRID:Addgene_49130)
For your References section:rAAV-compatible MiniPromoters for restricted expression in the brain and eye. de Leeuw CN, Korecki AJ, Berry GE, Hickmott JW, Lam SL, Lengyell TC, Bonaguro RJ, Borretta LJ, Chopra V, Chou AY, D'Souza CA, Kaspieva O, Laprise S, McInerny SC, Portales-Casamar E, Swanson-Newman MI, Wong K, Yang GS, Zhou M, Jones SJ, Holt RA, Asokan A, Goldowitz D, Wasserman WW, Simpson EM. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. 10.1186/s13041-016-0232-4 [pii] PubMed 27164903