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pBSFI-myr-akt-T308A/S473A
(Plasmid #49191)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49191 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBSFI
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 4500
  • Modifications to backbone
    pBSFI is based on pBluescript SK, with a different MCS inserted between SacI and KpnI
  • Vector type
    Adaptor Plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Akt1
  • Alt name
    Akt
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1500
  • Mutation
    T308A; S473A
  • GenBank ID
    NM_009652.3
  • Entrez Gene
    Akt1 (a.k.a. Akt, LTR-akt, PKB, PKB/Akt, PKBalpha, Rac)
  • Promoter T7
  • Tags / Fusion Proteins
    • myristolation signal (N terminal on insert)
    • HA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSFI-myr-akt-T308A/S473A was a gift from Peter Vogt (Addgene plasmid # 49191 ; http://n2t.net/addgene:49191 ; RRID:Addgene_49191)
  • For your References section:

    The akt kinase: molecular determinants of oncogenicity. Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5. 10.1073/pnas.95.25.14950 PubMed 9843996