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pIRES2-EGFP-deltaNp53:WTp53
(Plasmid #49244)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 49244 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIRES2-EGFP
  • Backbone manufacturer
    clonetch
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    p53 deltaN and p53 WT
  • Alt name
    TP53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2343
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter CMV
  • Tags / Fusion Proteins
    • His (C terminal on insert)
    • IRES-EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note there are some minor discrepancies between Addgene's quality control sequence and the depositor's assembled sequence. These differences are not known to affect function, though they may affect diagnostic digests.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES2-EGFP-deltaNp53:WTp53 was a gift from Dylan Taatjes (Addgene plasmid # 49244 ; http://n2t.net/addgene:49244 ; RRID:Addgene_49244)
  • For your References section:

    The human DeltaNp53 isoform triggers metabolic and gene expression changes that activate mTOR and alter mitochondrial function. Lin SC, Karoly ED, Taatjes DJ. Aging Cell. 2013 Oct;12(5):863-72. doi: 10.1111/acel.12108. Epub 2013 Jun 25. 10.1111/acel.12108 PubMed 23734707