PurposeChlamy Cp transformation for transgene integration via double-homologous recombination into the rbcL-psaB intergenic region
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||49342||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 2946
- Total vector size (bp) 8915
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameEcoRI 5.8 kB
Alt namep67 insert Chlamy Cp DNA
Insert Size (bp)5969
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer Universal forward
- 3′ sequencing primer Universal reverse (Common Sequencing Primers)
Terms and Licenses
There is 1bp mismatch (C to T) between the full reference sequence and Addgene's quality control sequence. The mismatch removes the NotI/EagI restriction site at position 670.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS-67Sma was a gift from Anastasios Melis (Addgene plasmid # 49342 ; http://n2t.net/addgene:49342 ; RRID:Addgene_49342)
For your References section:Marker-free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii. Chen HC, Melis A. Plant Biotechnol J. 2013 Sep;11(7):818-28. doi: 10.1111/pbi.12073. Epub 2013 May 7. 10.1111/pbi.12073 PubMed 23647698