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(Plasmid #49342)


Item Catalog # Description Quantity Price (USD)
Plasmid 49342 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 2946
  • Total vector size (bp) 8915
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    EcoRI 5.8 kB
  • Alt name
    p67 insert Chlamy Cp DNA
  • Species
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Universal forward
  • 3′ sequencing primer Universal reverse
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

There is 1bp mismatch (C to T) between the full reference sequence and Addgene's quality control sequence. The mismatch removes the NotI/EagI restriction site at position 670.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-67Sma was a gift from Anastasios Melis (Addgene plasmid # 49342 ; ; RRID:Addgene_49342)
  • For your References section:

    Marker-free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii. Chen HC, Melis A. Plant Biotechnol J. 2013 Sep;11(7):818-28. doi: 10.1111/pbi.12073. Epub 2013 May 7. 10.1111/pbi.12073 PubMed 23647698