Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #49342)


Item Catalog # Description Quantity Price (USD)
Plasmid 49342 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 2946
  • Total vector size (bp) 8915
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    EcoRI 5.8 kB
  • Alt name
    p67 insert Chlamy Cp DNA
  • Species
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer Universal forward
  • 3′ sequencing primer Universal reverse
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

There is 1bp mismatch (C to T) between the full reference sequence and Addgene's quality control sequence. The mismatch removes the NotI/EagI restriction site at position 670.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-67Sma was a gift from Anastasios Melis (Addgene plasmid # 49342 ; ; RRID:Addgene_49342)
  • For your References section:

    Marker-free genetic engineering of the chloroplast in the green microalga Chlamydomonas reinhardtii. Chen HC, Melis A. Plant Biotechnol J. 2013 Sep;11(7):818-28. doi: 10.1111/pbi.12073. Epub 2013 May 7. 10.1111/pbi.12073 PubMed 23647698