Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is operating at a reduced capacity.

  • Pending order? We will email to confirm that your organization can accept shipments.
  • Conducting coronavirus or COVID-19 research? Email us at [email protected] with your order or deposit number so we can prioritize it.

Learn more about our current shipping status and COVID-19 resources.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pLYS5-SDHB-Flag
(Plasmid #50055)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50055 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLYS5
  • Total vector size (bp) 8902
  • Modifications to backbone
    Flag tag C-terminal
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SDHB
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    831
  • GenBank ID
    NM_003000
  • Entrez Gene
    SDHB (a.k.a. CWS2, IP, PGL4, SDH, SDH1, SDH2, SDHIP)
  • Tag / Fusion Protein
    • Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Xma1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer custom
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLYS5-SDHB-Flag was a gift from Vamsi Mootha (Addgene plasmid # 50055 ; http://n2t.net/addgene:50055 ; RRID:Addgene_50055)
  • For your References section:

    EMRE is an Essential Component of the Mitochondrial Calcium Uniporter Complex. Sancak Y, Markhard AL, Kitami T, Kovacs-Bogdan E, Kamer KJ, Udeshi ND, Carr SA, Chaudhuri D, Clapham DE, Li AA, Calvo SE, Goldberger O, Mootha VK. Science. 2013 Nov 14. 10.1126/science.1242993 PubMed 24231807