PurposeExpresses Cph8 under a modified PLtetO-1 promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50552||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 1995
- Total vector size (bp) 4231
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)4230
Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter.
Requires pPLPCB(S)t or some other source of TetR
Requires PCB to function
Does NOT include the response regulator.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCph8 was a gift from Jeffrey Tabor (Addgene plasmid # 50552 ; http://n2t.net/addgene:50552 ; RRID:Addgene_50552)
For your References section:Characterizing bacterial gene circuit dynamics with optically programmed gene expression signals. Olson EJ, Hartsough LA, Landry BP, Shroff R, Tabor JJ. Nat Methods. 2014 Mar 9. doi: 10.1038/nmeth.2884. 10.1038/nmeth.2884 PubMed 24608181