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pAWH-Rluc-let-7-A114-N40-HhR
(Plasmid #50555)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50555 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAHW
  • Backbone manufacturer
    The Drosophila Gateway™ Vector Collection
  • Vector type
    Insect Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    mv1184
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Luciferase-let7-A114-N40-HhR
  • Species
    Synthetic
  • Promoter Actin5C

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Rluc gene derives from psiCheck2 from Promega.
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

We constructed a Renilla luciferase reporter for let-7 miRNA harboring A114-N40 (a protected poly(A) sequence internalized by a 40 nt unrelated sequence) followed by the hammerhead ribozyme sequence (HhR) that generates the 3' end by self-cleavage.

Unstable when transformed and so any retransformation must be checked by digest or sequencing before use. In strain MV1184, it is stable as a glycerol stock.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAWH-Rluc-let-7-A114-N40-HhR was a gift from Yukihide Tomari (Addgene plasmid # 50555 ; http://n2t.net/addgene:50555 ; RRID:Addgene_50555)
  • For your References section:

    MicroRNAs mediate gene silencing via multiple different pathways in drosophila. Fukaya T, Tomari Y. Mol Cell. 2012 Dec 28;48(6):825-36. doi: 10.1016/j.molcel.2012.09.024. Epub 2012 Nov 1. 10.1016/j.molcel.2012.09.024 PubMed 23123195