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miR-34aM53
(Plasmid #50830)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50830 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL3-Basic
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4818
  • Total vector size (bp) 6084
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miR-34A promoter M53
  • Alt name
    MIR34A
  • Alt name
    miR-34A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1266
  • Mutation
    p53 binding site mutated (-39)
  • Entrez Gene
    MIR34A (a.k.a. MIRN34A, miRNA34A, mir-34, mir-34a)
  • Promoter miR34a
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNrev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    miR-34aM53 was a gift from Yun Bai & Xueqing Xu (Addgene plasmid # 50830 ; http://n2t.net/addgene:50830 ; RRID:Addgene_50830)
  • For your References section:

    Transcriptional activation of microRNA-34a by NF-kappa B in human esophageal cancer cells. Li J, Wang K, Chen X, Meng H, Song M, Wang Y, Xu X, Bai Y. BMC Mol Biol. 2012 Jan 31;13:4. 10.1186/1471-2199-13-4 PubMed 22292433