Addgene diagnostic digest
This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 50977 with SmaI, BamHI-HF, and HindIII-HF.
We expected the following banding patterns:
1. SmaI single digest: Bands at 3.8 & 2.9 kb
2. BamHI-HF single digest: Linearized plasmid at 7.1kb
3. HindIII-HF single digest: Linearized plasmid at 7.1kb
4. BamHI-HF/ HindIII-HF double digest: Bands at 4.6 & 2.5 kb