pIKM1
(Plasmid #51054)

• Purpose: (Empty Backbone) Kanamycin and erethromycin resistance
• Depositing Labs: Lee Lynd, Juergen Wiegel
• Publication: Tyurin et al Appl Environ Microbiol. 2004 Feb;70(2):883-90.

Backbone

• Vector backbone: pIMP1
• Backbone manufacturer: Mermelstein et al. 1992
• Backbone size (bp): 4500
• Modifications to backbone: Added kanamycin resistance marker from pKD102
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Growth instructions: MLS marker gives resistance to erythromycin as well
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was a gift from Juergen Wiegel and is being submitted to Addgene with his approval.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

For more information about this plasmid, see the associated publication (PMID: 14766568), as well as the publication describing its original construction: Mai, Lorenz, and Wiegel, Transformation of Thermoanaerobacterium sp. strain JW/SL-YS485 with plasmid pIKM1 conferring kanamycin resistance. FEMS Microbiol Lett. 1997 DOI: 10.1111/j.1574-6968.1997.tb10283.x ( http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.1997.tb10283.x/abstract )