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pRGEB31
(Plasmid #51295)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51295 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAMBIA1300
  • Backbone manufacturer
    CAMBIA
  • Backbone size (bp) 8958
  • Modifications to backbone
    1. Removed Bsa I sites. 2. Inserted the U3-gRNA-35S-Cas9 cassette from pRGE31.
  • Vector type
    CRISPR ; plant expression
  • Promoter Rice snoRNA U3 and dual 35S promoter
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For agrobacterium strains like EHA105, growth temperature is 28 C.
  • Copy number
    High Copy

Cloning Information

Resource Information

Depositor Comments

The full plasmid sequence was assembled, the XmaI site is not present.

Please use the reference links above to access a published protocol for using plasmids pRGE31, pRGEB31, pRGE32 and pRGEB32 as well as a CRISPR design software tool.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRGEB31 was a gift from Yinong Yang (Addgene plasmid # 51295 ; http://n2t.net/addgene:51295 ; RRID:Addgene_51295)
  • For your References section:

    RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System. Xie K, Yang Y. Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 Aug 17. 10.1093/mp/sst119 PubMed 23956122