pET-Ce25
(Plasmid #51296)

• Purpose: Expresses a plant arginyl-tRNA synthetase in E.coli
• Depositing Lab: Gabor Igloi
• Publication: Hogg et al Protein Expr Purif. 2008 Oct;61(2):163-7. doi: 10.1016/j.pep.2008.05.008. Epub 2008 May 23.

Backbone

• Vector backbone: pET32a+
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5900
• Total vector size (bp): 7693
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: arginyl-tRNA synthetase
• Alt name: RARS
• Species: Canavalia ensiformis (jack bean)
• Insert Size (bp): 1791
• Mutation: GTT (Val) insertion after initiation codon to permit NcoI cloning
• GenBank ID: AM950325.1
• Promoter: T7
• Tags / Fusion Proteins:
  • Thioredoxin (N terminal on backbone)
  • His Tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • Purification of expressed enzyme

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For induction, use BL21CodonPlus, cool and grow at 23°C