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pMath3-luc
(Plasmid #51312)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51312 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Math3 promoter (-2.8k- +196)
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3000
  • GenBank ID
    AF034778.1
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HincII (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer F1ori-F
  • 3′ sequencing primer LucNRev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMath3-luc was a gift from Ryoichiro Kageyama (Addgene plasmid # 51312 ; http://n2t.net/addgene:51312 ; RRID:Addgene_51312)
  • For your References section:

    Structure and promoter analysis of Math3 gene, a mouse homolog of Drosophila proneural gene atonal. Neural-specific expression by dual promoter elements. Tsuda H, Takebayashi K, Nakanishi S, Kageyama R. J Biol Chem. 1998 Mar 13;273(11):6327-33. 10.1074/jbc.273.11.6327 PubMed 9497361