PurposeLuciferase reporter driven by the Math3 promoter
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51312||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonePica Gene basic vector
- Total vector size (bp) 5597
Growth in Bacteria
Gene/Insert nameMath3 promoter (-2.8k- +196)
SpeciesM. musculus (mouse)
Insert Size (bp)3000
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site HincII (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer F1ori-F
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMath3-luc was a gift from Ryoichiro Kageyama (Addgene plasmid # 51312 ; http://n2t.net/addgene:51312 ; RRID:Addgene_51312)
For your References section:Structure and promoter analysis of Math3 gene, a mouse homolog of Drosophila proneural gene atonal. Neural-specific expression by dual promoter elements. Tsuda H, Takebayashi K, Nakanishi S, Kageyama R. J Biol Chem. 1998 Mar 13;273(11):6327-33. 10.1074/jbc.273.11.6327 PubMed 9497361
Map uploaded by the depositor.