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pcDNA3-pri-miR-16-1
(Plasmid #51382)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51382 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIR16-1 microRNA 16-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Entrez Gene
    MIR16-1 (a.k.a. MIRN16-1, miRNA16-1, mir-16-1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CAATGGGCGTGGATAGCG
  • 3′ sequencing primer CCAGGGTCAAGGAAGGCAC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-pri-miR-16-1 was a gift from Narry Kim (Addgene plasmid # 51382 ; http://n2t.net/addgene:51382 ; RRID:Addgene_51382)
  • For your References section:

    Lin28 mediates the terminal uridylation of let-7 precursor MicroRNA. Heo I, Joo C, Cho J, Ha M, Han J, Kim VN. Mol Cell. 2008 Oct 24;32(2):276-84. doi: 10.1016/j.molcel.2008.09.014. 10.1016/j.molcel.2008.09.014 PubMed 18951094