PurposeExpresses truncated version of NLL-MetRS from endogenous MetG promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51401||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepQE80 kan
Modifications to backboneInsert containing the MetG promoter was inserted into the Nhe1 cut site in the backbone of the pQE80 vector.
Growth in Bacteria
Copy numberHigh Copy
MutationL13N, Y260L, H301L; Please note that a truncated version of MetRS is used for pAM1. The extra stop codon was deliberately put in the sequence and shortens the sequence from 677AA to 548AA.
Entrez GenemetG (a.k.a. b2114, ECK2107, JW2101)
- Promoter MetG
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (unknown if destroyed)
- 3′ cloning site Nhe1 (unknown if destroyed)
- 5′ sequencing primer 3primeUP
- 3′ sequencing primer T0term-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAM1 was a gift from David Tirrell (Addgene plasmid # 51401 ; http://n2t.net/addgene:51401 ; RRID:Addgene_51401)
For your References section:Identification of secreted bacterial proteins by noncanonical amino acid tagging. Mahdavi A, Szychowski J, Ngo JT, Sweredoski MJ, Graham RL, Hess S, Schneewind O, Mazmanian SK, Tirrell DA. Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):433-8. doi: 10.1073/pnas.1301740111. Epub 2013 Dec 17. 10.1073/pnas.1301740111 PubMed 24347637