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pGolden-Neo
(Plasmid #51422)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51422 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTopo
  • Backbone manufacturer
    lifetechnologies
  • Backbone size w/o insert (bp) 3519
  • Total vector size (bp) 5258
  • Modifications to backbone
    no
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PGK1 phosphoglycerate kinase 1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1737
  • Entrez Gene
    PGK1 (a.k.a. HEL-S-68p, MIG10, PGKA)
  • Promoter PGK

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer GGAAACAGCTATGACCATG
  • 3′ sequencing primer GTAAAACGACGGCCAGT
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The PGK-Neo-pA cassette was PCR amplified from the pNeDaKo-Neo vector from Bert Vogelstein and Kenneth W. Kinzler, John Hopkins University, Baltimore, US
  • Terms and Licenses
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGolden-Neo was a gift from Yonglun Luo (Addgene plasmid # 51422 ; http://n2t.net/addgene:51422 ; RRID:Addgene_51422)
  • For your References section:

    Efficient construction of rAAV-based gene targeting vectors by Golden Gate cloning. Luo Y, Lin L, Bolund L, Sorensen CB. Biotechniques. 2014 May 1;56(5):263-8. doi: 10.2144/000114169. eCollection 2014 May. 10.2144/000114169 PubMed 24806227