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PH-Btk-GFP
(Plasmid #51463)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51463 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 5253
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BTK (aa 1–177)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    531
  • GenBank ID
    NM_000061.2
  • Entrez Gene
    BTK (a.k.a. AGMX1, AT, ATK, BPK, IGHD3, IMD1, PSCTK1, XLA)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PH-Btk-GFP was a gift from Tamas Balla (Addgene plasmid # 51463 ; http://n2t.net/addgene:51463 ; RRID:Addgene_51463)
  • For your References section:

    Visualization of phosphoinositides that bind pleckstrin homology domains: calcium- and agonist-induced dynamic changes and relationship to myo-[3H]inositol-labeled phosphoinositide pools. Varnai P, Balla T. J Cell Biol. 1998 Oct 19;143(2):501-10. 10.1083/jcb.143.2.501 PubMed 9786958