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p2attNG-H11-long
(Plasmid #51545)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 51545 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p2attNG
  • Vector type
    Mammalian Expression ; Human Targeting
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    This plasmid may be difficult to grow and a strain such as MDS42 ΔrecA (Scarab Genomics C-0185) might increase yields.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP4m

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2attNG-H11-long was a gift from Michele Calos (Addgene plasmid # 51545 ; http://n2t.net/addgene:51545 ; RRID:Addgene_51545)
  • For your References section:

    DICE, an efficient system for iterative genomic editing in human pluripotent stem cells. Zhu F, Gamboa M, Farruggio AP, Hippenmeyer S, Tasic B, Schule B, Chen-Tsai Y, Calos MP. Nucleic Acids Res. 2013 Dec 4. 10.1093/nar/gkt1290 PubMed 24304893