PurposeConstitutively express endosome localized PhyB in Saccharomyces cerevisiae
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51574||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7236
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesA. thaliana (mustard weed)
Insert Size (bp)5755
Entrez GenePHYB (a.k.a. AT2G18790, HY3, MSF3.17, MSF3_17, OOP1, OUT OF PHASE 1, PHYTOCHROME B, phytochrome B)
- Promoter ADH1pr
/ Fusion Proteins
- mCherry (C terminal on insert)
- SNF7 (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Apa1 (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer mCherry F (Common Sequencing Primers)
There is a 15-aa linker (EFDSAGSAGSAGGSS) between the PhyB and mCherry and a 10-aa linker (SAGSAGKASG) between mCherry and anchor gene.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PhyB-mCherry-SNF7 was a gift from Chao Tang (Addgene plasmid # 51574)
For your References section:A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Yang X, Jost AP, Weiner OD, Tang C. Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 10.1091/mbc.E13-03-0126. Epub 2013 Jun 12. 10.1091/mbc.E13-03-0126 PubMed 23761071