D1433 his3::LYS2 Disruptor Converter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51676||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression ; yeast marker swap
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesS. cerevisiae (budding yeast)
Entrez GeneLYS2 (a.k.a. YBR115C)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Plasmid was made by cutting YDp-H with BglII and replacing the 60 bp BglII fragment with a 5 kb BamHI fragment (partial digest product) containing LYS2 from plasmid YDp-K.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:D1433 his3::LYS2 Disruptor Converter was a gift from David Stillman (Addgene plasmid # 51676 ; http://n2t.net/addgene:51676 ; RRID:Addgene_51676)
For your References section:New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Voth WP, Jiang YW, Stillman DJ. Yeast. 2003 Aug;20(11):985-93. 10.1002/yea.1018 PubMed 12898713