M3927 ura3::KanMX3 Disruptor Converter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51682||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression ; yeast marker swap
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site EcoRI (destroyed during cloning)
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Plasmid M3927 (ura3::KanMX3) was made by cutting YDp-U with EcoRV and inserting a 2.5 kb BglII–EcoRI (both ends blunted with Klenow) fragment containing KanMX3 from plasmid pFA6–KanMX3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:M3927 ura3::KanMX3 Disruptor Converter was a gift from David Stillman (Addgene plasmid # 51682 ; http://n2t.net/addgene:51682 ; RRID:Addgene_51682)
For your References section:New 'marker swap' plasmids for converting selectable markers on budding yeast gene disruptions and plasmids. Voth WP, Jiang YW, Stillman DJ. Yeast. 2003 Aug;20(11):985-93. 10.1002/yea.1018 PubMed 12898713