CH-GECO2.1
(Plasmid #52099)

• Purpose: mCherry-based Calcium indicators
• Depositing Lab: Robert Campbell
• Publication: Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763-72. doi: 10.1093/protein/gzt052. Epub 2013 Oct 22.

Backbone

• Vector backbone: pBAD/HisB
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 6388
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CH-GECO2.1
• Alt name: calcium indicator based on circular permutated mCherry
• Species: Synthetic; synthetic construct
• Insert Size (bp): 1266
• GenBank ID: KJ534664
• Promoter: pBAD promotor
• Tag / Fusion Protein:
  • 6 His tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI before 6xHis, NheI after 6xHis (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pBAD-Forward primer
• 3′ sequencing primer: pBAD-Reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry