|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32448||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backboneCustomized Vector
- Backbone size w/o insert (bp) 3200
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameblue intensiometric genetically encoded Ca2+-indicators for optical imaging
Insert Size (bp)1257
MutationGCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D305G/R377P/K380Q/ S404G
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer TAGAAGGCACAGTCGAGG (Common Sequencing Primers)
Note: Could not make stable cell line using this vector.
There is 1bp mismatch between Addgene's quality control sequence and the provided depositor's sequence, P5S in protein sequence. This mutation should not affect the protein function and the depositing lab confirmed that in vivo experiment before.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CMV-B-GECO1 was a gift from Robert Campbell (Addgene plasmid # 32448 ; http://n2t.net/addgene:32448 ; RRID:Addgene_32448)
For your References section:An Expanded Palette of Genetically Encoded Ca2+ Indicators. Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE. Science. 2011 Sep 8. 10.1126/science.1208592 PubMed 21903779