PurposeMammalian expression of blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32442||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneCustomized Vector
- Backbone size w/o insert (bp) 3200
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameemission ratiometric genetically encoded Ca2+-indicators for optical imaging
Insert Size (bp)1248
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer TAGAAGGCACAGTCGAGG (Common Sequencing Primers)
Note: Could not make stable cell line using this vector.
Addgene's sequencing result identified a single nucleotide mismatch at position 1182 when compared to the sequence provided by the depositing scientist and GenBank ID JN258409. According to the depositing scientist, this mismatch does not affect the protein sequence and is not a concern for the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CMV-GEM-GECO1 was a gift from Robert Campbell (Addgene plasmid # 32442)
For your References section:An Expanded Palette of Genetically Encoded Ca2+ Indicators. Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE. Science. 2011 Sep 8. 10.1126/science.1208592 PubMed 21903779