Addgene - CMV-GEM-GECO1 Plasmid Data

Plasmid 32442: CMV-GEM-GECO1

Gene/insert name:
GEM-GECO1
Alt name:
emission ratiometric genetically encoded Ca2+-indicators for optical imaging
Insert size:
1248
Species:
synthetic construct
GenBank ID:
JN258409
Mutation:
GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S/N302S/R377P/K380Q/S404G/E430V
Vector backbone:
Customized Vector
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
3200
Promoter:
CMV
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
TAATACGACTCACTATAGGG List of Sequencing Primers
3' sequencing primer:
TAGAAGGCACAGTCGAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Robert Campbell
Terms and Licenses:
MTA

Comments:

Note: Could not make stable cell line using this vector.

Addgene's sequencing result identified a single nucleotide mismatch at position 1182 when compared to the sequence provided by the depositing scientist and GenBank ID JN258409. According to the depositing scientist, this mismatch does not affect the protein sequence and is not a concern for the function of the plasmid.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An Expanded Palette of Genetically Encoded Ca2+ Indicators. Zhao et al (Science. 2011 Sep 8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32442" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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