Addgene - CMV-G-GECO1.0 Plasmid Data

Gene/insert name:
G-GECO1.0
Alt name:
green intensiometric genetically encoded Ca2+-indicators for optical imaging version 1.0
Insert size:
1251
GenBank ID:
JN258413
Mutation:
GCaMP3 K119I/L173Q/S404G/E430V
Vector backbone:
Customized Vector
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
3200
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
TAATACGACTCACTATAGGG List of Sequencing Primers
3' sequencing primer:
TAGAAGGCACAGTCGAGG
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH10B
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Robert Campbell
Terms and Licenses:
MTA

Comments:

Note: Could not make stable cell line using this vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An Expanded Palette of Genetically Encoded Ca2+ Indicators. Zhao et al (Science. 2011 Sep 8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 32447" in your Materials and Methods section.