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pTC201B
(Plasmid #52193)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52193 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEZ-Frt-lox-DT
  • Backbone manufacturer
    Klaus Rajewsky (Addgene plasmid # 11736)
  • Backbone size w/o insert (bp) 4500
  • Modifications to backbone
    The FRT-flanked cassette in pEZ was replaced by a synthetic fragment bearing an FRT-flanked splicing acceptor together with multiple cloning sites. A Neo-Ires-GFP-SV40 polyA fragment was inserted immediately downstream of the splicing acceptor to create the gene-trapping cassette. The left and right arms flanking the cassette, encompassing BAF57 exons 2 to 3 and exon 4, respectively, were amplified by PCR from C57B/6 mice
  • Vector type
    Cre/Lox
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    promoter-trap cassette Neo-Ires-GFP

Cloning Information

Resource Information

Depositor Comments

This plasmid carries the FRT-flanked promoter-Neo expression cassette, which is in turn flanked by LoxP site.

The targeted upstream trapped exon must be in frame with the Neo sequence in the vector in order to produce the exon-Neo fusion protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTC201B was a gift from Tian Chi (Addgene plasmid # 52193 ; http://n2t.net/addgene:52193 ; RRID:Addgene_52193)
  • For your References section:

    LoxP-FRT Trap (LOFT): a simple and flexible system for conventional and reversible gene targeting. Chaiyachati BH, Kaundal RK, Zhao J, Wu J, Flavell R, Chi T. BMC Biol. 2012 Nov 30;10:96. doi: 10.1186/1741-7007-10-96. 10.1186/1741-7007-10-96 PubMed 23198860