PurposeConstruct used for viral production of virus expressing the TVA receptor, Rabies protein G and a marker protein TagBFP.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52425||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5340
- Total vector size (bp) 8615
Vector typeAAV, Cre/Lox ; ; Adeno-Associated Virus
Growth in Bacteria
Growth instructionsStbl2 E coli from Invitrogen Grow at 30 degrees in 2xYT media
Copy numberLow Copy
Gene/Insert nameTagBFP, TVA receptor, RabiesG
- Promoter CAG
- Cloning method Unknown
- 5′ sequencing primer tcacaagcgcgtttcacctcctg
- 3′ sequencing primer gtcccgcaagccctttt (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV-FLEX-rev-BFP-2a-TVA-2a-RabiesG was a gift from Scott Sternson (Addgene plasmid # 52425)
For your References section:Parallel, redundant circuit organization for homeostatic control of feeding behavior. Betley JN, Cao ZF, Ritola KD, Sternson SM. Cell. 2013 Dec 5;155(6):1337-50. doi: 10.1016/j.cell.2013.11.002. 10.1016/j.cell.2013.11.002 PubMed 24315102