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pDEST pcDNA5-FRT/TO-eGFP-Rif1
(Plasmid #52506)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52506 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST pcDNA5/FRT/TO-eGFP
  • Backbone size w/o insert (bp) 7510
  • Total vector size (bp) 14906
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RIF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    7396
  • Mutation
    siRNA resistant sequence: ATAGTAC
  • Entrez Gene
    RIF1
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Resource Information

Depositor Comments

The Rif1 insert contains G836S, V1362M, N2021Y, L2392V variants compared to the NCBI reference for isoform 2 [NM_001177663.1] but matches the NCBI protein sequence AAV51401.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST pcDNA5-FRT/TO-eGFP-Rif1 was a gift from Daniel Durocher (Addgene plasmid # 52506 ; http://n2t.net/addgene:52506 ; RRID:Addgene_52506)
  • For your References section:

    A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and BRCA1-CtIP controls DNA repair pathway choice. Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D. Mol Cell. 2013 Mar 7;49(5):872-83. doi: 10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17. 10.1016/j.molcel.2013.01.001 PubMed 23333306