pDEST pcDNA5-FRT/TO-eGFP-Rif1
(Plasmid #52506)

• Purpose: mammalian expression vector of Rif1 siRNA resistant
• Depositing Lab: Daniel Durocher
• Publication: Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi: 10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.

Backbone

• Vector backbone: pDEST pcDNA5/FRT/TO-eGFP
• Backbone size w/o insert (bp): 7510
• Total vector size (bp): 14906
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl2
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RIF1
• Species: H. sapiens (human)
• Insert Size (bp): 7396
• Mutation: siRNA resistant sequence: ATAGTAC
• Entrez Gene: RIF1
• Tag / Fusion Protein:
  • eGFP (N terminal on backbone)

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Rif1 insert contains G836S, V1362M, N2021Y, L2392V variants compared to the NCBI reference for isoform 2 [NM_001177663.1] but matches the NCBI protein sequence AAV51401.1.