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pJM31
(Plasmid #52986)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52986 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pPD 49.83
  • Backbone size w/o insert (bp) 4710
  • Total vector size (bp) 6648
  • Modifications to backbone
    duplicated unc-54 3' utr
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Annexin V
  • Species
    H. sapiens (human)
  • Entrez Gene
    ANXA5 (a.k.a. ANX5, ENX2, HEL-S-7, PP4, RPRGL3)
  • Promoter hsp-16.41
  • Tags / Fusion Proteins
    • GFP (w/ introns) (C terminal on insert)
    • signal sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer hsp-16
  • 3′ sequencing primer unc-54
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJM31 was a gift from Ding Xue (Addgene plasmid # 52986 ; http://n2t.net/addgene:52986 ; RRID:Addgene_52986)
  • For your References section:

    CED-1, CED-7, and TTR-52 regulate surface phosphatidylserine expression on apoptotic and phagocytic cells. Mapes J, Chen YZ, Kim A, Mitani S, Kang BH, Xue D. Curr Biol. 2012 Jul 24;22(14):1267-75. doi: 10.1016/j.cub.2012.05.052. Epub 2012 Jun 21. 10.1016/j.cub.2012.05.052 PubMed 22727702