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pHL 1303
(Plasmid #53008)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53008 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    unknown
  • Backbone manufacturer
    Lim lab
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    sodB
  • Alt name
    target RNA
  • Species
    E.coli
  • Mutation
    contains -56 to +141 relative to start codon
  • Promoter pLtetO-1
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    ompC
  • Species
    E. coli
  • Mutation
    contains −81 to +36 bp relative to start codon
  • Promoter pLlacO-1
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHL 1303 was a gift from Han Lim (Addgene plasmid # 53008 ; http://n2t.net/addgene:53008 ; RRID:Addgene_53008)
  • For your References section:

    Disruption of small RNA signaling caused by competition for Hfq. Hussein R, Lim HN. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. 10.1073/pnas.1010082108 PubMed 21189298