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SNV-memTdTomato-2AcytoGFP
(Plasmid #53124)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53124 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBMN-LACZ
  • Backbone manufacturer
    Nolan Lab
  • Vector type
    Retroviral ; expression in chicken cells
  • Promoter SNV (spleen necrosis virus)
  • Tags / Fusion Proteins
    • memTdTomato (C terminal on insert)
    • 2A-cyto-GFP (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is unstable during transformation. If doing a fresh transformation, confirm correct size by digest before proceeding further (~9kb).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SNV-memTdTomato-2AcytoGFP was a gift from Jeanette Hyer (Addgene plasmid # 53124 ; http://n2t.net/addgene:53124 ; RRID:Addgene_53124)
  • For your References section:

    Murine retroviruses re-engineered for lineage tracing and expression of toxic genes in the developing chick embryo. Venters SJ, Dias da Silva MR, Hyer J. Dev Dyn. 2008 Nov;237(11):3260-9. doi: 10.1002/dvdy.21766. 10.1002/dvdy.21766 PubMed 18942139