Purpose(Empty Backbone) Retrovirus vector backbone that co-expresses GFP and a gene of interest fused to memTdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53124||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerNolan Lab
Vector typeRetroviral ; expression in chicken cells
- Promoter SNV (spleen necrosis virus)
/ Fusion Proteins
- memTdTomato (C terminal on insert)
- 2A-cyto-GFP (C terminal on insert)
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBMN5'
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid is unstable during transformation. If doing a fresh transformation, confirm correct size by digest before proceeding further (~9kb).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SNV-memTdTomato-2AcytoGFP was a gift from Jeanette Hyer (Addgene plasmid # 53124 ; http://n2t.net/addgene:53124 ; RRID:Addgene_53124)
For your References section:Murine retroviruses re-engineered for lineage tracing and expression of toxic genes in the developing chick embryo. Venters SJ, Dias da Silva MR, Hyer J. Dev Dyn. 2008 Nov;237(11):3260-9. doi: 10.1002/dvdy.21766. 10.1002/dvdy.21766 PubMed 18942139